In vitro radiolabeling procedure which labels the proteins of Newcastle disease virions with carbon-14
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چکیده
منابع مشابه
In vitro radiolabeling procedure which labels the proteins of Newcastle disease virions with carbon-14.
A convenient and gentle procedure was used for in vitro radioisotopic labeling the proteins of Newcastle disease virus. This method, which utilizes [14C]formaldehyde and sodium borohydride, labels all the proteins of purified Newcastle disease virus. Virions labeled in this manner retained biological and biopysical integrity
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Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase.
The virions of Newcastle disease virus (NDV) contained an enzyme that catalyzed the incorporation of ribonucleotides into ribonucleic acid (RNA). Optimal conditions for this polymerase activity were identical to the conditions for the vesicular stomatitis virus (VSV) polymerase, and both enzymes were active for longer times at 32 C than at 37 C. However, the specific activity of the NDV polymer...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1975
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.12.6.1411-1414.1975